验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, A375 cells, A431 cells, Jurkat cells, U-937 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach tissue, human colon cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16278-1-AP targets CHMP6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, canine |
| 免疫原 | CHMP6 fusion protein Ag9357 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromatin modifying protein 6 |
| 别名 | Vps20, Vacuolar protein sorting-associated protein 20, hVps20, Chromatin-modifying protein 6, chromatin modifying protein 6 |
| 计算分子量 | 201 aa, 23 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC010108 |
| 基因名称 | CHMP6 |
| Gene ID (NCBI) | 79643 |
| RRID | AB_2079498 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96FZ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP6 antibody 16278-1-AP | Download protocol |
| IHC protocol for CHMP6 antibody 16278-1-AP | Download protocol |
| IP protocol for CHMP6 antibody 16278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
Curr Biol A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. | ||
Nat Commun Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy
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