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验证数据展示

  • WB analysis using 68406-1-Ig

    WB analysis using 68406-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68406-1-Ig (CHMP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human prostate cancer using 68406-1-Ig

    IHC staining of human prostate cancer using 68406-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 68406-1-Ig

    IHC staining of human prostate cancer using 68406-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68406-1-Ig

    IF Staining of A431 using 68406-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (68406-1-Ig, Clone: 2F8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CHMP7 Monoclonal antibody

CHMP7 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68406-1-Ig

产品说明书

CloneNo. 2F8F6

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CHMP family, member 7, CHMP7, Chromatin modifying protein 7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68406-1-Ig targets CHMP7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 CHMP7 fusion protein Ag9641 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CHMP family, member 7
别名 CHMP family, member 7, CHMP7, Chromatin modifying protein 7
计算分子量 453 aa, 51 kDa
观测分子量 51-55 kDa
GenBank蛋白编号BC019110
基因名称 CHMP7
Gene ID (NCBI) 91782
RRIDAB_3085123
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WUX9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHMP7 antibody 68406-1-IgDownload protocol
IHC protocol for CHMP7 antibody 68406-1-IgDownload protocol
IF protocol for CHMP7 antibody 68406-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols