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CHN1 Polyclonal antibody

CHN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12048-1-AP

Print datasheet

Synonyms

A chimaerin, Alpha chimerin, ARHGAP2, chimerin (chimaerin) 1, CHN, CHN1, DURS2, N chimaerin, N chimerin, NC, RHOGAP2

验证数据展示

  • WB analysis using 12048-1-AP

    WB analysis using 12048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12048-1-AP (CHN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12048-1-AP

    IHC staining of human gliomas using 12048-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, PC-12 cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12048-1-AP targets CHN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CHN1 fusion protein Ag2678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chimerin (chimaerin) 1
Synonyms A chimaerin, Alpha chimerin, ARHGAP2, chimerin (chimaerin) 1, CHN, CHN1, DURS2, N chimaerin, N chimerin, NC, RHOGAP2
Calculated Molecular Weight 459 aa, 53 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC011393
Gene Symbol CHN1
Gene ID (NCBI) 1123
RRIDAB_10794446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15882
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHN1 antibody 12048-1-APDownload protocol
IHC protocol for CHN1 antibody 12048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis.

Authors - Jianbing Liu
View Article
humanWB,IHC

Bioengineered

Bioinformatics-based analysis of the association between the A1-chimaerin (CHN1) gene and gastric cancer

Authors - Jie-Pin Li
View Article