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验证数据展示

  • WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

    WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

    WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

    Un-treated and Tunicamycin treated HepG2 lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDIT3 antibody included
  • IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDIT3 antibody included

CHOP/GADD153 Monoclonal antibody, PBS Only

CHOP/GADD153 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66741-1-PBS

产品说明书

CloneNo. 4F3G1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CHOP; GADD153, CHOP, DDIT3, GADD153, 4F3G1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.

产品信息

66741-1-PBS targets CHOP/GADD153 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHOP/GADD153 fusion protein Ag7354 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DNA-damage-inducible transcript 3
别名 CHOP; GADD153, CHOP, DDIT3, GADD153, 4F3G1
计算分子量 19 kDa
观测分子量 30 kDa
GenBank蛋白编号BC003637
基因名称 CHOP
Gene ID (NCBI) 1649
RRIDAB_2882089
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35638
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).