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验证数据展示

  • WB analysis using 25525-1-AP

    WB analysis using 25525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25525-1-AP (CHORDC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25525-1-AP

    IF Staining of HEK-293 using 25525-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CHORDC1 antibody (25525-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CHORDC1 Polyclonal antibody

CHORDC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25525-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CHP-1, CHP1, CHP 1, CHORD-containing protein 1, CHORD domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25525-1-AP targets CHORDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CHORDC1 fusion protein Ag18315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteine and histidine-rich domain (CHORD)-containing 1
别名 CHP-1, CHP1, CHP 1, CHORD-containing protein 1, CHORD domain-containing protein 1
计算分子量 332 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC072461
基因名称 CHORDC1
Gene ID (NCBI) 26973
RRIDAB_2918091
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHD1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHORDC1 antibody 25525-1-APDownload protocol
IF protocol for CHORDC1 antibody 25525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols