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Chordin Polyclonal antibody

Chordin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27293-1-AP

Print datasheet

Synonyms

chordin, CHRD

验证数据展示

  • WB analysis of 3T3-L1 using 27293-1-AP

    WB analysis of 3T3-L1 using 27293-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27293-1-AP (CHRD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27293-1-AP

    IHC staining of human liver cancer using 27293-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 27293-1-AP

    IHC staining of human liver cancer using 27293-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected in3T3-L1 cells
Positive IHC detected inhuman liver cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

27293-1-AP targets Chordin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Chordin fusion protein Ag26001 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chordin
Synonyms chordin, CHRD
Calculated Molecular Weight 955 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC112345
Gene Symbol Chordin
Gene ID (NCBI) 8646
RRIDAB_2880832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2X0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration.

实验方案

Product Specific Protocols
WB protocol for Chordin antibody 27293-1-APDownload protocol
IHC protocol for Chordin antibody 27293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Oncogene

Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis

Authors - Jing Ma
View Article