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  • Featured Product
  • KD/KO Validated

CHRNA7/CHRFAM7A Polyclonal antibody

CHRNA7/CHRFAM7A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21586-1-AP

Print datasheet

Synonyms

CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10

验证数据展示

  • WB analysis of Jurkat using 21586-1-AP

    WB analysis of Jurkat using 21586-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 21586-1-AP

    WB analysis of Jurkat using 21586-1-AP

    WB result of CHRNA7/CHRFAM7A antibody (21586-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7/CHRFAM7A transfected Jurkat cells.

经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21586-1-AP targets CHRNA7/CHRFAM7A in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CHRNA7/CHRFAM7A fusion protein Ag16151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
Synonyms CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10
Calculated Molecular Weight 412 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC101348
Gene Symbol CHRNA7/CHRFAM7A
Gene ID (NCBI) 89832
RRIDAB_10734326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ494W8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal acetylcholine receptor subunit alpha-7, encoded by the CHRNA7 gene, is involved in cognition through interneuron modulation of 4-(2-aminoethyl)benzene-1,2-diol and glutamate signaling. CHRNA7 gene is genetically linked to multiple disorders with cognitive deficits. A partial duplication of CHRNA7 (CHRFAM7A) is found in humans. CHRFAM7A gene encodes the CHRNA7-FAM7A fusion protein. Expression of CHRFAM7A has been reported in brain, peripheral blood lymphocytes (PBLs), and a broad range of epithelial cells (PMID: 21718690; 23553139; 25681457). CHRFAM7A has been associated with many neuropsychiatric disorders (PMID: 25906356; 25701707). This antibody, raised against 241-386 amino acids of human CHRFAM7A protein, recognizes both CHRNA7 and CHRFAM7A.

实验方案

Product Specific Protocols
WB protocol for CHRNA7/CHRFAM7A antibody 21586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols