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CHRNA4 Polyclonal antibody

CHRNA4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21939-1-AP

Print datasheet

Synonyms

BFNC, CHRNA4, EBN, EBN1, NACHR, NACHRA4, NACRA4

验证数据展示

  • WB analysis using 21939-1-AP

    WB analysis using 21939-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21939-1-AP (CHRNA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNeuro-2a cells, C6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21939-1-AP targets CHRNA4 in WB, ELISA applications and shows reactivity with human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, Mouse
Immunogen CHRNA4 fusion protein Ag16584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 4
Synonyms BFNC, CHRNA4, EBN, EBN1, NACHR, NACHRA4, NACRA4
Calculated Molecular Weight 627 aa, 70 kDa
Observed Molecular Weight69 kDa
GenBank Accession NumberBC096290
Gene Symbol CHRNA4
Gene ID (NCBI) 1137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1.

实验方案

Product Specific Protocols
WB protocol for CHRNA4 antibody 21939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols