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验证数据展示

  • WB analysis of MCF-7 using 60220-1-Ig

    WB analysis of MCF-7 using 60220-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 60220-1-Ig (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 60220-1-Ig

    IHC staining of human brain using 60220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60220-1-Ig (CHRNA7 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of human brain using 60220-1-Ig

    IHC staining of human brain using 60220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60220-1-Ig (CHRNA7 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CHRNA7 antibody included

CHRNA7 Monoclonal antibody

CHRNA7 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60220-1-Ig

产品说明书

CloneNo. 2H1G3
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, ELISA

别名

CHRNA7, CHRNA7 2, NACHRA7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHRNA7 Ready-To-Use IHC Kit. CHRNA7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60220-1-Ig targets CHRNA7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CHRNA7 fusion protein Ag15980 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cholinergic receptor, nicotinic, alpha 7
别名 CHRNA7, CHRNA7 2, NACHRA7
计算分子量 502 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC037571
基因名称 CHRNA7
Gene ID (NCBI) 1139
RRIDAB_11043180
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin.

实验方案

Product Specific Protocols
WB protocol for CHRNA7 antibody 60220-1-IgDownload protocol
IHC protocol for CHRNA7 antibody 60220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Functional Human α7 Nicotinic Acetylcholine Receptor (nAChR) Generated from E. coli.

Authors - Tommy S Tillman
View Article