CHRNB1 Polyclonal antibody

CHRNB1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11553-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman liver tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

11553-1-AP targets CHRNB1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 CHRNB1 fusion protein Ag2108 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cholinergic receptor, nicotinic, beta 1 (muscle)
别名 ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS
计算分子量 501 aa, 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号BC023553
基因名称 CHRNB1
Gene ID (NCBI) 1140
RRIDAB_2291854
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11230
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acetylcholine receptors (AChRs) are integral membrane proteins that respond to the binding of acetylcholine (ACh), a neurotransmitter synthesized, stored and finally released by cholinergic neurons. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) subunits. CHRNB1 gene encodes acetylcholine receptor subunit beta (ACHRB). Mutations in this gene are associated with slow-channel congenital myasthenic syndrome.

实验方案

Product Specific Protocols
WB protocol for CHRNB1 antibody 11553-1-APDownload protocol
IHC protocol for CHRNB1 antibody 11553-1-APDownload protocol
IP protocol for CHRNB1 antibody 11553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
mouseWB

Aging (Albany NY)

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Authors - Dongdong Wang
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo
mouseWB

Genes Immun

Plasticity of the murine spleen T-cell cholinergic receptors and their role in in vitro differentiation of na?ve CD4 T cells toward the Th1, Th2 and Th17 lineages.

Authors - Qian J J
mouseWB

Biochem Biophys Res Commun

Association of a newly identified lncRNA LNC_000280 with the formation of acetylcholine receptor clusters in vitro.

Authors - Jin Deng
mouseWB

Mol Ther Nucleic Acids

MiR-142a-3p: A novel ACh receptor transcriptional regulator in association with peripheral nerve injury

Authors - Zhidan Qi
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