验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
11553-1-AP targets CHRNB1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | CHRNB1 fusion protein Ag2108 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cholinergic receptor, nicotinic, beta 1 (muscle) |
| Synonyms | ACHRB, CHRNB, CHRNB1, CMS1D, CMS2A, SCCMS |
| Calculated Molecular Weight | 501 aa, 57 kDa |
| Observed Molecular Weight | 50-57 kDa |
| GenBank Accession Number | BC023553 |
| Gene Symbol | CHRNB1 |
| Gene ID (NCBI) | 1140 |
| RRID | AB_2291854 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11230 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acetylcholine receptors (AChRs) are integral membrane proteins that respond to the binding of acetylcholine (ACh), a neurotransmitter synthesized, stored and finally released by cholinergic neurons. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) subunits. CHRNB1 gene encodes acetylcholine receptor subunit beta (ACHRB). Mutations in this gene are associated with slow-channel congenital myasthenic syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHRNB1 antibody 11553-1-AP | Download protocol |
| IHC protocol for CHRNB1 antibody 11553-1-AP | Download protocol |
| IP protocol for CHRNB1 antibody 11553-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients. | ||
Aging (Albany NY) Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis. | ||
Transl Psychiatry A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota. | ||
Genes Immun Plasticity of the murine spleen T-cell cholinergic receptors and their role in in vitro differentiation of na?ve CD4 T cells toward the Th1, Th2 and Th17 lineages. | ||
Biochem Biophys Res Commun Association of a newly identified lncRNA LNC_000280 with the formation of acetylcholine receptor clusters in vitro. | ||
Mol Ther Nucleic Acids MiR-142a-3p: A novel ACh receptor transcriptional regulator in association with peripheral nerve injury |
