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CHST14 Polyclonal antibody

CHST14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17749-1-AP

Print datasheet

Synonyms

CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST

验证数据展示

  • WB analysis of HEK-293 using 17749-1-AP

    WB analysis of HEK-293 using 17749-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17749-1-AP (CHST14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 17749-1-AP

    IHC staining of human placenta using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 17749-1-AP

    IHC staining of human placenta using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17749-1-AP targets CHST14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CHST14 fusion protein Ag12014 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
Synonyms CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST
Calculated Molecular Weight 376 aa, 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC049214
Gene Symbol CHST14
Gene ID (NCBI) 113189
RRIDAB_2079051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHST14 antibody 17749-1-APDownload protocol
IHC protocol for CHST14 antibody 17749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Transl Oncol

A comprehensive analysis of the expression, immune infiltration, prognosis and partial experimental validation of CHST family genes in gastric cancer

Authors - Jinbo Zhan
View Article