• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CHST9 Polyclonal antibody

CHST9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13135-1-AP

Print datasheet

Synonyms

UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2

验证数据展示

  • WB analysis of HepG2 using 13135-1-AP

    WB analysis of HepG2 using 13135-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13135-1-AP (CHST9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13135-1-AP (CHST9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13135-1-AP targets CHST9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHST9 fusion protein Ag3874 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9
Synonyms UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2
Calculated Molecular Weight 438 aa, 52 kDa
Observed Molecular Weight 52 kDa, 42 kDa
GenBank Accession NumberBC025764
Gene Symbol CHST9
Gene ID (NCBI) 83539
RRIDAB_2079222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L1S5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHST9 antibody 13135-1-APDownload protocol
IHC protocol for CHST9 antibody 13135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols