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验证数据展示

  • WB analysis of HepG2 using 13135-1-AP

    WB analysis of HepG2 using 13135-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13135-1-AP (CHST9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13135-1-AP (CHST9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHST9 Polyclonal antibody

CHST9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13135-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13135-1-AP targets CHST9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHST9 fusion protein Ag3874 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9
别名 UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2
计算分子量 438 aa, 52 kDa
观测分子量 52 kDa, 42 kDa
GenBank蛋白编号BC025764
基因名称 CHST9
Gene ID (NCBI) 83539
RRIDAB_2079222
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L1S5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHST9 antibody 13135-1-APDownload protocol
IHC protocol for CHST9 antibody 13135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols