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验证数据展示

  • WB analysis using 13030-1-AP

    WB analysis using 13030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13030-1-AP (CHTF18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 13030-1-AP

    IHC staining of human medulloblastoma using 13030-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 13030-1-AP (CHTF18 antibody) at dilution of 1:100 (under 10x lens).

CHTF18 Polyclonal antibody

CHTF18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13030-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, CHTF18, Ctf18, hCTF18, RUVBL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13030-1-AP targets CHTF18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHTF18 fusion protein Ag3672 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
别名 C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, CHTF18, Ctf18, hCTF18, RUVBL
计算分子量 1184 aa, 129 kDa
观测分子量 110 kDa
GenBank蛋白编号BC018184
基因名称 CHTF18
Gene ID (NCBI) 63922
RRIDAB_2260469
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVB6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHTF18 antibody 13030-1-APDownload protocol
IHC protocol for CHTF18 antibody 13030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols