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CHTF18 Polyclonal antibody

CHTF18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13030-1-AP

Print datasheet

Synonyms

C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, CHTF18, Ctf18, hCTF18, RUVBL

验证数据展示

  • WB analysis using 13030-1-AP

    WB analysis using 13030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13030-1-AP (CHTF18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 13030-1-AP

    IHC staining of human medulloblastoma using 13030-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 13030-1-AP (CHTF18 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13030-1-AP targets CHTF18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHTF18 fusion protein Ag3672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Synonyms C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, CHTF18, Ctf18, hCTF18, RUVBL
Calculated Molecular Weight 1184 aa, 129 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC018184
Gene Symbol CHTF18
Gene ID (NCBI) 63922
RRIDAB_2260469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHTF18 antibody 13030-1-APDownload protocol
IHC protocol for CHTF18 antibody 13030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols