验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13170-1-AP targets CIDEA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CIDEA fusion protein Ag3824 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell death-inducing DFFA-like effector a |
| 别名 | Cell death activator CIDE A, CIDE A, CIDEA |
| 计算分子量 | 253 aa, 28 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC031896 |
| 基因名称 | CIDEA |
| Gene ID (NCBI) | 1149 |
| RRID | AB_10642957 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60543 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cell death-inducing DNA fragmentation factor (DFFA)-like effector (CIDE) family proteins, including Cidea, Cideb, and Fsp27 (Cidec), are lipid droplet (LD)-associated proteins playing important roles in LD fusion, lipid secretion, and very-low-density-lipoprotein (VLDL) maturation. Cidea is highly enriched in adult brown adipose tissue (BAT), and its deficiency results in the accumulation of smaller LDs in brown adipocytes, improved INS sensitivity, and resistance to diet-induced obesity. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIDEA antibody 13170-1-AP | Download protocol |
| IHC protocol for CIDEA antibody 13170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development. | ||
EMBO J Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues. | ||
Diabetes Fat-Specific Knockout of Mecp2 Upregulates Slpi to Reduce Obesity by Enhancing Browning. | ||
J Agric Food Chem Polysaccharide from Ziyang Selenium-Enriched Green Tea Prevents Obesity and Promotes Adipose Thermogenesis via Modulating the Gut Microbiota | ||
Food Funct Artemisia sphaerocephala Krasch polysaccharide promotes adipose thermogenesis and decreases obesity by shaping the gut microbiota |
