验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
27600-1-AP targets CIDEB in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CIDEB fusion protein Ag27003 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cell death-inducing DFFA-like effector b |
| Synonyms | Cell death activator CIDE B, CIDEB |
| Calculated Molecular Weight | 219 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC035970 |
| Gene Symbol | CIDEB |
| Gene ID (NCBI) | 27141 |
| RRID | AB_2880922 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cell death‐inducing DFFA‐like effector B (CIDEB), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although CIDEA is expressed at high levels in brown adipose tissue (BAT) and CIDEC is highly enriched in white adipose tissue (WAT) and BAT, CIDEB is more abundant in liver, kidney, small intestine, and colon. CIDEB is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIDEB antibody 27600-1-AP | Download protocol |
| IHC protocol for CIDEB antibody 27600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis. | ||
Cancer Biol Ther The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer | ||
Int J Mol Sci Unveiling Novel Mechanism of CIDEB in Fatty Acid Synthesis Through ChIP-Seq and Functional Analysis in Dairy Goat |
