验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 1 publications below |
产品信息
12287-1-AP targets CIDEC in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | CIDEC fusion protein Ag2913 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell death-inducing DFFA-like effector c |
| 别名 | Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27 |
| 计算分子量 | 238 aa, 27 kDa |
| 观测分子量 | 27-35 kDa |
| GenBank蛋白编号 | BC016851 |
| 基因名称 | CIDEC |
| Gene ID (NCBI) | 63924 |
| RRID | AB_2877842 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cell death-inducing DFF45-like effector (CIDE) proteins, including CIDEA, CIDEB and CIDEC (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. CIDEC was found to be highly expressed in adipose tissues and to function as a lipid droplet-binding protein that promotes lipid accumulation in adipocytes. While normal mouse livers express low levels of CIDEC protein, its expression is highly upregulated in NALFD, but not in chronic ethanol feeding-induced fatty liver. (PMID: 26099526) Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIDEC antibody 12287-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Nutr Biochem Identification of potential pathogenic hepatic super-enhancers regulatory network in high-fat diet induced hyperlipidemia | ||
Commun Biol Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression. | ||
Cancer Biol Ther The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer | ||
Biomed Pharmacother Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway. | ||
Aging Cell Long-term NAD+ supplementation prevents the progression of age-related hearing loss in mice | ||
Cell Death Discov Shift work promotes adipogenesis via cortisol-dependent downregulation of EGR3-HDAC6 pathway |
