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CIN85 Polyclonal antibody

CIN85 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12132-1-AP

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Synonyms

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3



经过测试的应用

Positive WB detected inRaji cells, fetal human brain tissue, Ramos cells, Jurkat cells, THP-1 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12132-1-AP targets CIN85 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CIN85 fusion protein Ag2777 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3-domain kinase binding protein 1
Synonyms SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3
Calculated Molecular Weight 665 aa, 73 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC015806
Gene Symbol SH3KBP1/CIN85
Gene ID (NCBI) 30011
RRIDAB_2187277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96B97
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

实验方案

Product Specific Protocols
WB protocol for CIN85 antibody 12132-1-APDownload protocol
IHC protocol for CIN85 antibody 12132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.

Authors - Ihor Yakymovych
humanWB

Biol Cell

ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.

Authors - Yoon Hye-Young HY
  • KD Validated
humanIHC

Arch Gynecol Obstet

Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.

Authors - Ma Yaxi Y