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CIN85 Polyclonal antibody

CIN85 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12132-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRaji cells, fetal human brain tissue, Ramos cells, Jurkat cells, THP-1 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12132-1-AP targets CIN85 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 CIN85 fusion protein Ag2777 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3-domain kinase binding protein 1
别名 SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3
计算分子量 665 aa, 73 kDa
观测分子量 85 kDa
GenBank蛋白编号BC015806
基因名称 SH3KBP1/CIN85
Gene ID (NCBI) 30011
RRIDAB_2187277
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

实验方案

Product Specific Protocols
WB protocol for CIN85 antibody 12132-1-APDownload protocol
IHC protocol for CIN85 antibody 12132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.

Authors - Ihor Yakymovych
humanWB

Biol Cell

ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.

Authors - Yoon Hye-Young HY
  • KD Validated
humanIHC

Arch Gynecol Obstet

Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.

Authors - Ma Yaxi Y
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