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- KD/KO Validated
CIN85 Polyclonal antibody
CIN85 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, fetal human brain tissue, Ramos cells, Jurkat cells, THP-1 cells, NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12132-1-AP targets CIN85 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | CIN85 fusion protein Ag2777 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SH3-domain kinase binding protein 1 |
| Synonyms | CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1 |
| Calculated Molecular Weight | 665 aa, 73 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC015806 |
| Gene Symbol | SH3KBP1/CIN85 |
| Gene ID (NCBI) | 30011 |
| RRID | AB_2187277 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96B97 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIN85 antibody 12132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface. | ||
Biol Cell ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.
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