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Cirhin Polyclonal antibody

Cirhin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11057-1-AP

Print datasheet

Synonyms

CIRH1A, Cirhin, KIAA1988, NAIC, TEX292, UTP4

验证数据展示

  • WB analysis using 11057-1-AP

    WB analysis using 11057-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11057-1-AP (Cirhin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 11057-1-AP

    IP experiment of HepG2 using 11057-1-AP

    IP result of anti-Cirhin (IP:11057-1-AP, 4ug; Detection:11057-1-AP 1:700) with HepG2 cells lysate 2600 ug.

  • IHC staining of mouse brain using 11057-1-AP

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11057-1-AP

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11057-1-AP targets Cirhin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Cirhin fusion protein Ag1494 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cirrhosis, autosomal recessive 1A (cirhin)
Synonyms CIRH1A, Cirhin, KIAA1988, NAIC, TEX292, UTP4
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 70-77 kDa
GenBank Accession NumberBC009348
Gene Symbol Cirhin
Gene ID (NCBI) 84916
RRIDAB_2877743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969X6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cirhin is a protein that in humans is encoded by the CIRH1A gene. Mutation of this gene has been associated with North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence (PMID: 12417987). Catalog#11057-1-AP is a rabbit polyclonal antibody raised against the full-length of human CIRHIN.

实验方案

Product Specific Protocols
WB protocol for Cirhin antibody 11057-1-APDownload protocol
IHC protocol for Cirhin antibody 11057-1-APDownload protocol
IP protocol for Cirhin antibody 11057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols