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验证数据展示

  • WB analysis using 11057-1-AP

    WB analysis using 11057-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11057-1-AP (Cirhin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 11057-1-AP

    IP experiment of HepG2 using 11057-1-AP

    IP result of anti-Cirhin (IP:11057-1-AP, 4ug; Detection:11057-1-AP 1:700) with HepG2 cells lysate 2600 ug.

  • IHC staining of mouse brain using 11057-1-AP

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11057-1-AP

    IHC staining of mouse brain using 11057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11057-1-AP (Cirhin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cirhin Polyclonal antibody

Cirhin Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11057-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

CIRH1A, Cirhin, KIAA1988, NAIC, TEX292, UTP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11057-1-AP targets Cirhin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cirhin fusion protein Ag1494 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cirrhosis, autosomal recessive 1A (cirhin)
别名 CIRH1A, Cirhin, KIAA1988, NAIC, TEX292, UTP4
计算分子量 77 kDa
观测分子量 70-77 kDa
GenBank蛋白编号BC009348
基因名称 Cirhin
Gene ID (NCBI) 84916
RRIDAB_2877743
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969X6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cirhin is a protein that in humans is encoded by the CIRH1A gene. Mutation of this gene has been associated with North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence (PMID: 12417987). Catalog#11057-1-AP is a rabbit polyclonal antibody raised against the full-length of human CIRHIN.

实验方案

Product Specific Protocols
WB protocol for Cirhin antibody 11057-1-APDownload protocol
IHC protocol for Cirhin antibody 11057-1-APDownload protocol
IP protocol for Cirhin antibody 11057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols