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CITED2 Polyclonal antibody

CITED2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15021-1-AP

Print datasheet

Synonyms

MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1

验证数据展示

  • WB analysis of SH-SY5Y using 15021-1-AP

    WB analysis of SH-SY5Y using 15021-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 15021-1-AP

    IF Staining of SH-SY5Y using 15021-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15021-1-AP (CITED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15021-1-AP targets CITED2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CITED2 fusion protein Ag7078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
Synonyms MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 36 kDa, 27 kDa
GenBank Accession NumberBC004377
Gene Symbol CITED2
Gene ID (NCBI) 10370
RRIDAB_2080545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99967
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].

实验方案

Product Specific Protocols
WB protocol for CITED2 antibody 15021-1-APDownload protocol
IF protocol for CITED2 antibody 15021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Toxicol Sci

The role of miR-182-5p in hepatocarcinogenesis of trichloroethylene in mice.

Authors - Yan Jiang
View Article