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Ch-TOG Recombinant antibody, PBS Only (Detector)

Ch-TOG Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241546G8

Cat no : 84285-3-PBS

Print datasheet

Synonyms

CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG

验证数据展示

  • WB analysis using 84285-3-RR (same clone as 84285-3-PBS)

    WB analysis using 84285-3-RR (same clone as 84285-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84285-3-RR (Ch-TOG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 84285-3-RR (same clone as 84285-3-PBS)

    IHC staining of mouse cerebellum using 84285-3-RR (same clone as 84285-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84285-3-RR (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • IHC staining of mouse cerebellum using 84285-3-RR (same clone as 84285-3-PBS)

    IHC staining of mouse cerebellum using 84285-3-RR (same clone as 84285-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84285-3-RR (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP5/Ch-TOG antibody included
  • FC experiment of HeLa using 84285-3-RR (same clone as 84285-3-PBS)

    FC experiment of HeLa using 84285-3-RR (same clone as 84285-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ckap5 Recombinant Antibody (84285-3-RR, Clone:241546G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84285-3-RR (same clone as 84285-3-PBS)

    FC experiment of A549 using 84285-3-RR (same clone as 84285-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Ckap5 Recombinant Antibody (84285-3-RR, Clone:241546G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84285-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01203-1

    Cytometric bead array standard curve of MP01203-1

    Cytometric bead array standard curve of MP01203-1, Ch-TOG Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84285-1-PBS. Detection antibody: 84285-3-PBS. Standard: Ag24875. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 84285-3-RR

    Affinity and Kinetic Characterization of 84285-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84285-3-RR against Human Ch-TOG were performed. The affinity constant is 1.05 nM.

Planning an IHC experiment? We recommend our IHCeasy CKAP5/Ch-TOG Ready-To-Use IHC Kit. CKAP5/Ch-TOG primary antibody included.

产品信息

84285-3-PBS targets Ch-TOG as part of a matched antibody pair:

MP01203-1: 84285-1-PBS capture and 84285-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Ch-TOG fusion protein Ag24875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytoskeleton associated protein 5
Synonyms CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC120869
Gene Symbol Ch-TOG
Gene ID (NCBI) 9793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14008
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.