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验证数据展示

  • WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

    WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKB antibody included
  • IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKB antibody included
  • Featured Product
  • KD/KO验证

CKB Monoclonal antibody, PBS Only

CKB Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66764-1-PBS

产品说明书

CloneNo. 2B7C3

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse

应用

WB, IHC, Indirect ELISA

别名

B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CKB Ready-To-Use IHC Kit. CKB primary antibody included.

产品信息

66764-1-PBS targets CKB in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse
免疫原 CKB fusion protein Ag7933 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 creatine kinase, brain
别名 B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC001190
基因名称 CKB
Gene ID (NCBI) 1152
RRIDAB_2882110
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12277
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.