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  • Featured Product
  • KD/KO Validated

CKB Monoclonal antibody, PBS Only

CKB Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

2B7C3

Cat No : 66764-1-PBS

Print datasheet

Synonyms

B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain

验证数据展示

  • WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

    WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKB antibody included
  • IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKB antibody included
Planning an IHC experiment? We recommend our IHCeasy CKB Ready-To-Use IHC Kit. CKB primary antibody included.

产品信息

66764-1-PBS targets CKB in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human, Mouse
Immunogen CKB fusion protein Ag7933 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name creatine kinase, brain
Synonyms B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001190
Gene Symbol CKB
Gene ID (NCBI) 1152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12277
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.