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验证数据展示

  • IHC staining of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

    IHC staining of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 15891-1-AP (same clone as 15891-1-PBS)

    IHC staining of rat heart using 15891-1-AP (same clone as 15891-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

    WB analysis of mouse heart using 15891-1-AP (same clone as 15891-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15891-1-PBS in a different storage buffer formulation.

CKM/CKB Polyclonal antibody, PBS Only

CKM/CKB Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15891-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15891-1-PBS targets CKM/CKB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CKM/CKB fusion protein Ag8678 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 creatine kinase, muscle
别名
计算分子量 381 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC007462
基因名称 CKM
Gene ID (NCBI) 1158
RRIDAB_2260647
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06732
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.