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CKMT1A Polyclonal antibody

CKMT1A Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15346-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IP, ELISA and More (1)

Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat heart tissue, human colon tissue, human testis tissue, mouse heart tissue, mouse testis tissue, HEK-293 cells, A549 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15346-1-AP targets CKMT1A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 CKMT1A fusion protein Ag7583 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 creatine kinase, mitochondrial 1A
别名 Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi
计算分子量 47 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC001926
基因名称 CKMT1A
Gene ID (NCBI) 548596
RRIDAB_2081073
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12532
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

实验方案

Product Specific Protocols
WB protocol for CKMT1A antibody 15346-1-APDownload protocol
IHC protocol for CKMT1A antibody 15346-1-APDownload protocol
IP protocol for CKMT1A antibody 15346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

Authors - Kiran Kurmi
  • KD Validated
humanWB,IHC,IF

Nat Commun

Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.

Authors - Liang-Jie Wang
  • KD Validated
humanIHC, WB

Respir Res

Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.

Authors - Ming Li
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
mouseIHC

Int J Cancer

Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients.

Authors - Kenichiro Enooku
ratIHC

Front Cell Neurosci

Investigations into photoreceptor energy metabolism during experimental retinal detachment

Authors - Glyn Chidlow
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