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CKMT1A Recombinant antibody

CKMT1A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240825A4

Cat no : 83750-4-RR

Print datasheet

Synonyms

CKMT1B, CKMT1, CKMT, CKMiMi, Acidic-type mitochondrial creatine kinase

验证数据展示

  • WB analysis using 83750-4-RR

    WB analysis using 83750-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83750-4-RR (CKMT1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83750-4-RR

    Affinity and Kinetic Characterization of 83750-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83750-4-RR against Human CKMT1A were performed. The affinity constant is 2.86 nM.

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, SH-SY5Y cells, mouse heart tissue, rat heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83750-4-RR is CKMT1A Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CKMT1A fusion protein Ag7583 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name creatine kinase, mitochondrial 1A
Synonyms CKMT1B, CKMT1, CKMT, CKMiMi, Acidic-type mitochondrial creatine kinase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC001926
Gene Symbol CKMT1A
Gene ID (NCBI) 548596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

实验方案

Product Specific Protocols
WB protocol for CKMT1A antibody 83750-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols