验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13207-1-AP targets CKMT2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 | CKMT2 fusion protein Ag3971 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | creatine kinase, mitochondrial 2 (sarcomeric) |
| 别名 | Mib-CK, Mib CK, EC:2.7.3.2, Creatine kinase S-type, mitochondrial, CKMT 2 |
| 计算分子量 | 419 aa, 48 kDa |
| 观测分子量 | 41-48 kDa |
| GenBank蛋白编号 | BC029140 |
| 基因名称 | CKMT2 |
| Gene ID (NCBI) | 1160 |
| RRID | AB_2081188 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17540 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CKMT2 antibody 13207-1-AP | Download protocol |
| IHC protocol for CKMT2 antibody 13207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity. | ||
Mol Cell Proteomics Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens | ||
Metabolism Sustained mitochondrial biogenesis is essential to maintain caloric restriction-induced beige adipocytes. | ||
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
Mol Cell Proteomics Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens. | ||
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. |
