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CLASP1 Polyclonal antibody

CLASP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20803-1-AP

Print datasheet

Synonyms

CLASP1, CLIP associating protein 1, hOrbit1, KIAA0622, MAST1, Multiple asters homolog 1, Protein Orbit homolog 1

验证数据展示

  • WB analysis using 20803-1-AP

    WB analysis using 20803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20803-1-AP

    IHC staining of human testis using 20803-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 20803-1-AP

    IHC staining of human testis using 20803-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 20803-1-AP

    WB analysis using 20803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 20803-1-AP

    IP experiment of HeLa using 20803-1-AP

    IP result of anti-CLASP1 (IP:20803-1-AP, 4ug; Detection:20803-1-AP 1:2000) with HeLa cells lysate 1750 ug.

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20803-1-AP targets CLASP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CLASP1 fusion protein Ag14724 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytoplasmic linker associated protein 1
Synonyms CLASP1, CLIP associating protein 1, hOrbit1, KIAA0622, MAST1, Multiple asters homolog 1, Protein Orbit homolog 1
Calculated Molecular Weight 1538 aa, 169 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberBC112940
Gene Symbol CLASP1
Gene ID (NCBI) 23332
RRIDAB_10697808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z460
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLASP1 is a member of CLASP family. Members of the CLASP family of proteins are involved in multiple MT-dependent processes, including Golgi complex organization and trafficking, kinetochore fiber dynamics and flux, mitotic spindle assembly, and the stabilization of MT plus ends near the cortex. CLASP proteins regulate MT dynamics by promoting MT rescue and suppressing MT catastrophe. Recent work showed that CLASP1 activity is required for proper positioning of the mitotic spindle in both the C. elegans embryo and mammalian cells. Recent work has also identified roles CLASP1 in the regulation of spindle positioning.

实验方案

Product Specific Protocols
WB protocol for CLASP1 antibody 20803-1-APDownload protocol
IHC protocol for CLASP1 antibody 20803-1-APDownload protocol
IP protocol for CLASP1 antibody 20803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols