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CLCA1 Polyclonal antibody, PBS Only

CLCA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25291-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 25291-1-AP (same clone as 25291-1-PBS)

    WB analysis using 25291-1-AP (same clone as 25291-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25291-1-AP (CLCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 25291-1-AP (same clone as 25291-1-PBS)

    IHC staining of human small intestine using 25291-1-AP (same clone as 25291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25291-1-AP (CLCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25291-1-PBS in a different storage buffer formulation.

产品信息

25291-1-PBS targets CLCA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CLCA1 fusion protein Ag18176 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chloride channel accessory 1
Synonyms
Calculated Molecular Weight 914 aa, 100 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC156805
Gene Symbol CLCA1
Gene ID (NCBI) 1179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA8K7I4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calcium-activated chloride channel regulator 1(CLCA1) is a secreted protein, and it has an N-terminal zinc-dependent metallohydrolase domain with a conserved HExxE catalytic motif similar to that found in matrix metalloproteases (MMPs), and "a Disintegrin and metalloproteases" (ADAMs). The only known substrate for CLCA1 is CLCA1 itself as it undergoes intracellular autocatalytic cleavage resulting in two cleavage products which are both secreted. Since a previous study suggested that a band around 75 kDa, corresponding to the N-terminal autocatalytic cleavage product, was observed in mucus from both human and WT mice. In addition, an additional band at 50 kDa was identified in human and WT mouse mucus, suggesting that CLCA1 can be further processed. However, no band corresponding to the full-length un-cleaved CLCA1 could be detected in the human samples, and only a very faint band was observed in WT mouse mucus, indicating that most of the CLCA1 that is secreted into the mucus is fully cleaved in vivo. (PMID: 29885864)