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CLCN5 Polyclonal antibody

CLCN5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26812-1-AP

Print datasheet

Synonyms

chloride channel 5, Chloride channel protein 5, Chloride transporter ClC 5, ClC 5, CLC5, CLCK2, CLCN5, DENTS, hCIC K2, hClC K2, XLRH, XRN

验证数据展示

  • WB analysis of MDCK using 26812-1-AP

    WB analysis of MDCK using 26812-1-AP

    MDCK cells were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 26812-1-AP

    IHC staining of human kidney using 26812-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26812-1-AP

    IHC staining of human kidney using 26812-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inMDCK cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26812-1-AP targets CLCN5 in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, Canine, mouse
Cited Reactivitymouse
Immunogen CLCN5 fusion protein Ag25182 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chloride channel 5
Synonyms chloride channel 5, Chloride channel protein 5, Chloride transporter ClC 5, ClC 5, CLC5, CLCK2, CLCN5, DENTS, hCIC K2, hClC K2, XLRH, XRN
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC130429
Gene Symbol CLCN5
Gene ID (NCBI) 1184
RRIDAB_2880643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51795
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLCN5 antibody 26812-1-APDownload protocol
IHC protocol for CLCN5 antibody 26812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Gene Ther

Gene therapy of Dent disease type 1 in newborn ClC-5 null mice for sustained transgene expression and gene therapy effects

Authors - Pin Lyu
View Article