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CLCN7 Polyclonal antibody

CLCN7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29230-1-AP

Print datasheet

Synonyms

OPTA2, H(+)/Cl(-) exchange transporter 7, ClC-7, CLC7, CLC 7

验证数据展示

  • WB analysis using 29230-1-AP

    WB analysis using 29230-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29230-1-AP (CLCN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse kidney tissue, mouse small intestine mouse small intestine, rat small intestine rat small intestine

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29230-1-AP targets CLCN7 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CLCN7 fusion protein Ag29882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chloride channel 7
Synonyms OPTA2, H(+)/Cl(-) exchange transporter 7, ClC-7, CLC7, CLC 7
Calculated Molecular Weight 805 aa, 89 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC012737
Gene Symbol CLCN7
Gene ID (NCBI) 1186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The intracellular CLC chloride channel family members ClC-3, ClC-4, and ClC-5 have been proposed as the primary endosomal chloride conductance providers. ClC-7 is expressed in late endosomes and lysosomes, but there is disagreement in the literature regarding its contribution to acidification. More recently, the cystic fibrosis (CF) transmembrane conductance regulator chloride channel (CFTR) was proposed as the specific counter-ion conductance necessary for lysosomal acidification in alveolar macrophages. (PMID: 20566682)

实验方案

Product Specific Protocols
WB protocol for CLCN7 antibody 29230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols