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验证数据展示

  • WB analysis using 14402-1-AP

    WB analysis using 14402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 14402-1-AP

    IP experiment of HEK-293 using 14402-1-AP

    IP result of anti-CLCNKA (IP:14402-1-AP, 5ug; Detection:14402-1-AP 1:500) with HEK-293 cells lysate 880ug.

  • IHC staining of human kidney using 14402-1-AP

    IHC staining of human kidney using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 14402-1-AP

    IHC staining of human kidney using 14402-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLCNKA Polyclonal antibody

CLCNKA Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14402-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue, mouse pancreas tissue, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14402-1-AP targets CLCNKA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLCNKA fusion protein Ag5647 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chloride channel Ka
别名 chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka
计算分子量 75 kDa
观测分子量 70 kDa
GenBank蛋白编号BC048282
基因名称 CLCNKA
Gene ID (NCBI) 1187
RRIDAB_2079857
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51800
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLCNKA antibody 14402-1-APDownload protocol
IHC protocol for CLCNKA antibody 14402-1-APDownload protocol
IP protocol for CLCNKA antibody 14402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols