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CLEC16A Polyclonal antibody

CLEC16A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26257-1-AP

Print datasheet

Synonyms

CLEC16A, Gop 1, KIAA0350, Protein CLEC16A

验证数据展示

  • IHC staining of human tonsillitis using 26257-1-AP

    IHC staining of human tonsillitis using 26257-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26257-1-AP (CLEC16A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 26257-1-AP

    IHC staining of human tonsillitis using 26257-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26257-1-AP (CLEC16A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse spleen using 26257-1-AP

    WB analysis of mouse spleen using 26257-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26257-1-AP (CLEC16A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26257-1-AP is CLEC16A Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CLEC16A fusion protein Ag24268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C-type lectin domain family 16, member A
Synonyms CLEC16A, Gop 1, KIAA0350, Protein CLEC16A
Calculated Molecular Weight 1053 aa, 118 kDa
Observed Molecular Weight 104-110 kDa
GenBank Accession NumberBC112897
Gene Symbol CLEC16A
Gene ID (NCBI) 23274
RRIDAB_2880449
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2KHT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLEC16A antibody 26257-1-APDownload protocol
IHC protocol for CLEC16A antibody 26257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols