• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CLEC1A Polyclonal antibody

CLEC1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13394-1-AP

Print datasheet

Synonyms

C type lectin like receptor 1, CLEC 1, CLEC1, CLEC1A

验证数据展示

  • WB analysis using 13394-1-AP

    WB analysis using 13394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13394-1-AP (CLEC1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 13394-1-AP

    IHC staining of mouse lung using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13394-1-AP (CLEC1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 13394-1-AP

    IHC staining of mouse lung using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13394-1-AP (CLEC1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse thymus tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13394-1-AP targets CLEC1A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CLEC1A fusion protein Ag4058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C-type lectin domain family 1, member A
Synonyms C type lectin like receptor 1, CLEC 1, CLEC1, CLEC1A
Calculated Molecular Weight 280 aa, 32 kDa
Observed Molecular Weight 50 kDa, 32 kDa
GenBank Accession NumberBC039072
Gene Symbol CLEC1A
Gene ID (NCBI) 51267
RRIDAB_2083443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC1A (C-type lectin domain family 1 member A) is a single-pass type II membrane protein that contains one C-type lectin domain. Members of the C-type lectin domain family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC1A is expressed preferentially in dendritic cells and plays a role in regulating dendritic cell function (PMID: 10671229).

实验方案

Product Specific Protocols
WB protocol for CLEC1A antibody 13394-1-APDownload protocol
IHC protocol for CLEC1A antibody 13394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

EMBO Mol Med

Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation

Authors - Wei Jiang
View Article
mouse,humanIF

Invest Ophthalmol Vis Sci

CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis.

Authors - Guoqiang Zhu
View Article