验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
12814-1-AP targets CLEC2/CLEC1B in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CLEC2/CLEC1B fusion protein Ag3512 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | C-type lectin domain family 1, member B |
| 别名 | 1810061I13Rik, C type lectin like receptor 2, CLEC 2, CLEC1B, CLEC2, CLEC2B, PRO1384, QDED721 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 32-38 kDa |
| GenBank蛋白编号 | BC029554 |
| 基因名称 | CLEC2 |
| Gene ID (NCBI) | 51266 |
| RRID | AB_3085400 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P126 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C-type lectin-like receptor 2 (CLEC2, also known as CLEC1B) is a type II transmembrane receptor of the C-type lectin superfamily, which are characterized by one or more C-type lectin-like domains (CTLDs) (PMID: 10671229; 34177942). CLEC2 contains a single YXXL/hemi-ITAM (immuno-receptor tyrosine-based activation motif) within its cytoplasmic domain. CLEC-2 is highly expressed on platelets and megakaryocytes and at low levels on other hematopoietic cells, including dendritic cells, neutrophils, monocytes, and macrophages (PMID: 21728173). The first identified ligand for CLEC2 was the platelet-aggregating snake venom protein rhodocytin, and podoplanin has been established as an endogenous ligand for CLEC2 (PMID: 26151067). CLEC2 is involved in thrombosis/hemostasis, tumor metastasis, and lymphangiogenesis (PMID: 20525685). CLEC2 can be glycosylated and has been detected as 32- and 40-kDa forms in platelets with varying degrees of glycosylation (PMID: 16174766; 25150298; 21781241).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC2/CLEC1B antibody 12814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation | ||
Int J Gen Med CLEC1B is a Promising Prognostic Biomarker and Correlated with Immune Infiltration in Hepatocellular Carcinoma. |