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CLEC3B Polyclonal antibody

CLEC3B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20311-1-AP

Print datasheet

Synonyms

TNA, TN, Tetranectin, Plasminogen kringle 4-binding protein, C-type lectin domain family 3 member B

验证数据展示

  • WB analysis of human plasma using 20311-1-AP

    WB analysis of human plasma using 20311-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20311-1-AP (CLEC3B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of human plasma using 20311-1-AP

    IP experiment of human plasma using 20311-1-AP

    IP result of anti-CLEC3B (IP:20311-1-AP, 4ug; Detection:20311-1-AP 1:300) with human plasma lysate 4000ug.

  • IF Staining of HeLa using 20311-1-AP

    IF Staining of HeLa using 20311-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLEC3B antibody (20311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20311-1-AP targets CLEC3B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CLEC3B fusion protein Ag14147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C-type lectin domain family 3, member B
Synonyms TNA, TN, Tetranectin, Plasminogen kringle 4-binding protein, C-type lectin domain family 3 member B
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC011024
Gene Symbol CLEC3B
Gene ID (NCBI) 7123
RRIDAB_2878670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-Type Lectin Domain Family 3 Member B (CLEC3B) is a transmembrane Ca2+-binding protein, locating in cell plasma, extracellular matrix and exosomes. Downregulation of CLEC3B has been reported in various diseases. It was demonstrated in CLEC3B-deficient mice that the absence of CLEC3B impeded the mineralization process in osteogenesis. (PMID: 31477130)

实验方案

Product Specific Protocols
WB protocol for CLEC3B antibody 20311-1-APDownload protocol
IF protocol for CLEC3B antibody 20311-1-APDownload protocol
IP protocol for CLEC3B antibody 20311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols