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验证数据展示

  • IHC staining of human liver using 18173-1-AP

    IHC staining of human liver using 18173-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18173-1-AP (CLEC4G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 18173-1-AP

    IHC staining of human liver using 18173-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18173-1-AP (CLEC4G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver using 18173-1-AP

    IF Staining of human liver using 18173-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CLEC4G antibody (18173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human liver using 18173-1-AP

    IF Staining of human liver using 18173-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CLEC4G antibody (18173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CLEC4G Polyclonal antibody

CLEC4G Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 18173-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IHC, IF-P, ELISA and More (1)

别名

CLEC4G, LP2698, LSECtin, UNQ431

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

18173-1-AP targets CLEC4G in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性mouse
免疫原 CLEC4G fusion protein Ag12837 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-type lectin domain family 4, member G
别名 CLEC4G, LP2698, LSECtin, UNQ431
计算分子量 293 aa, 33 kDa
观测分子量 40 kDa
GenBank蛋白编号BC093691
基因名称 CLEC4G
Gene ID (NCBI) 339390
RRIDAB_2878512
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXB4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC4G, also known as LSECtin, is a member of the C-type lectin family of glycan-binding receptors that is expressed on sinusoidal endothelial cells in liver and lymph nodes. CLEC4G is a type II transmembrane protein of ∼40 kDa in size with a single C-type lectin-like domain at the COOH terminus, closest in homology to DC-SIGNR, DC-SIGN, and CD23. CLEC4G has been reported as a receptor for Japanese encephalitis virus (PubMed:24623090), ebolavirus (PubMed:16051304), SARS coronavirus/SARS-CoV (PubMed:16051304), lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).

实验方案

Product Specific Protocols
IHC protocol for CLEC4G antibody 18173-1-APDownload protocol
IF protocol for CLEC4G antibody 18173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

JHEP Rep

Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver

Authors - Kiryu K Yap
View Article