Calmegin Polyclonal antibody
Calmegin Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
12629-1-AP targets Calmegin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | Calmegin fusion protein Ag3322 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | calmegin |
| Synonyms | calmegin, CLGN |
| Calculated Molecular Weight | 610 aa, 70 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC028357 |
| Gene Symbol | Calmegin |
| Gene ID (NCBI) | 1047 |
| RRID | AB_2276364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14967 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calmegin antibody 12629-1-AP | Download protocol |
| IHC protocol for Calmegin antibody 12629-1-AP | Download protocol |
| IP protocol for Calmegin antibody 12629-1-AP | Download protocol |
| FC protocol for Calmegin antibody 12629-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis. | ||
