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CLIC1 Polyclonal antibody
CLIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human small intestine tissue, human colon tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, Hela cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14545-1-AP targets CLIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | CLIC1 fusion protein Ag6049 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | chloride intracellular channel 1 |
Synonyms | NCC27, G6 |
Calculated Molecular Weight | 27 kDa |
Observed Molecular Weight | 27-32 kDa |
GenBank Accession Number | BC064527 |
Gene Symbol | CLIC1 |
Gene ID (NCBI) | 1192 |
RRID | AB_2081809 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00299 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CLIC1 antibody 14545-1-AP | Download protocol |
IHC protocol for CLIC1 antibody 14545-1-AP | Download protocol |
IF protocol for CLIC1 antibody 14545-1-AP | Download protocol |
IP protocol for CLIC1 antibody 14545-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. | ||
Transl Oncol Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling
| ||
Cell Biochem Biophys CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway. | ||
Anticancer Res Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma. | ||
Technol Cancer Res Treat CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.
| ||
Breast Cancer (Dove Med Press) Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer |