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CLIC4 Polyclonal antibody

CLIC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12298-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

12298-2-AP targets CLIC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CLIC4 fusion protein Ag2942 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chloride intracellular channel 4
别名 CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1
计算分子量 253 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC012444
基因名称 CLIC4
Gene ID (NCBI) 25932
RRIDAB_2081969
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y696
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

实验方案

Product Specific Protocols
WB protocol for CLIC4 antibody 12298-2-APDownload protocol
IHC protocol for CLIC4 antibody 12298-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biomedicines

CLIC4 Is a New Biomarker for Glioma Prognosis

Authors - Zhichun Liu
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