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CLIC4 Polyclonal antibody

CLIC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12298-2-AP

Print datasheet

Synonyms

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

验证数据展示

  • WB analysis of HeLa using 12298-2-AP

    WB analysis of HeLa using 12298-2-AP

    WB result of CLIC4 antibody (12298-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

  • WB analysis of mouse kidney using 12298-2-AP

    WB analysis of mouse kidney using 12298-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 12298-2-AP

    IHC staining of human kidney using 12298-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 12298-2-AP

    IHC staining of human kidney using 12298-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12298-2-AP targets CLIC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CLIC4 fusion protein Ag2942 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chloride intracellular channel 4
Synonyms CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1
Calculated Molecular Weight 253 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC012444
Gene Symbol CLIC4
Gene ID (NCBI) 25932
RRIDAB_2081969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y696
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

实验方案

Product Specific Protocols
WB protocol for CLIC4 antibody 12298-2-APDownload protocol
IHC protocol for CLIC4 antibody 12298-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols