验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CLIP1 Ready-To-Use IHC Kit. CLIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23839-1-AP targets CLIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CLIP1 fusion protein Ag20811 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CAP-GLY domain containing linker protein 1 |
| 别名 | RSN, Reed-Sternberg intermediate filament-associated protein, Cytoplasmic linker protein 170 alpha-2, CYLN1, CLIP-170 |
| 计算分子量 | 1438 aa, 162 kDa |
| 观测分子量 | 160-170 kDa, 135 kDa |
| GenBank蛋白编号 | BC126305 |
| 基因名称 | CLIP1 |
| Gene ID (NCBI) | 6249 |
| RRID | AB_2879334 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P30622 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLIP1 antibody 23839-1-AP | Download protocol |
| IF protocol for CLIP1 antibody 23839-1-AP | Download protocol |
| IP protocol for CLIP1 antibody 23839-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. | ||
Cell Death Dis Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration | ||
Ecotoxicol Environ Saf Exosomal circCLIP1 regulates PM2.5-induced airway obstruction via targeting SEPT10 in vitro | ||
Exp Mol Med Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver |
