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CLIP4 Polyclonal antibody

CLIP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17044-1-AP

Print datasheet

Synonyms

RSNL2, Restin-like protein 2, CAP-Gly domain-containing linker protein 4

验证数据展示

  • WB analysis of HUVEC using 17044-1-AP

    WB analysis of HUVEC using 17044-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 17044-1-AP (CLIP4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 17044-1-AP

    WB analysis of Y79 using 17044-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 17044-1-AP (CLIP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 17044-1-AP

    IF Staining of HUVEC using 17044-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 17044-1-AP (CLIP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHUVEC cells, Y79 cells
Positive IF/ICC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17044-1-AP targets CLIP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CLIP4 fusion protein Ag10590 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CAP-GLY domain containing linker protein family, member 4
Synonyms RSNL2, Restin-like protein 2, CAP-Gly domain-containing linker protein 4
Calculated Molecular Weight 705 aa, 76 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberBC015310
Gene Symbol CLIP4
Gene ID (NCBI) 79745
RRIDAB_2878341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3C7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLIP4 antibody 17044-1-APDownload protocol
IF protocol for CLIP4 antibody 17044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

miR-135b-5p promotes gastric carcinogenesis by targeting CLIP4-mediated JAK2/STAT3 signal pathway

Authors - Zhiwei Peng
View Article