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验证数据展示

  • WB analysis using 20386-1-AP

    WB analysis using 20386-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20386-1-AP (CLN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20386-1-AP

    IHC staining of human brain using 20386-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20386-1-AP (CLN3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 20386-1-AP

    IHC staining of human brain using 20386-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20386-1-AP (CLN3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20386-1-AP

    IF Staining of HepG2 using 20386-1-AP

    Immunofluorescent analysis of HepG2 cells, using CLN3 antibody 20386-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CLN3 Polyclonal antibody

CLN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20386-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Protein CLN3, JNCL, BTS, BATTENIN, Batten disease protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, SH-SY5Y cells, Raji cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20386-1-AP targets CLN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLN3 fusion protein Ag14224 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ceroid-lipofuscinosis, neuronal 3
别名 Protein CLN3, JNCL, BTS, BATTENIN, Batten disease protein
计算分子量 438 aa, 48 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC002394
基因名称 CLN3
Gene ID (NCBI) 1201
RRIDAB_10694819
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803).

实验方案

Product Specific Protocols
WB protocol for CLN3 antibody 20386-1-APDownload protocol
IHC protocol for CLN3 antibody 20386-1-APDownload protocol
IF protocol for CLN3 antibody 20386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols