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验证数据展示

  • WB analysis of SH-SY5Y using 20315-1-AP

    WB analysis of SH-SY5Y using 20315-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20315-1-AP (CLN6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20315-1-AP

    IP experiment of HEK-293 using 20315-1-AP

    IP result of anti-CLN6 (IP:20315-1-AP, 4ug; Detection:20315-1-AP 1:300) with HEK-293 cells lysate 2560ug.

  • IHC staining of human colon using 20315-1-AP

    IHC staining of human colon using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 20315-1-AP

    IHC staining of human colon using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 20315-1-AP

    IF Staining of HEK-293 using 20315-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20315-1-AP (CLN6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CLN6 Polyclonal antibody

CLN6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20315-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CLN6, HsT18960, nclf, Protein CLN6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20315-1-AP targets CLN6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 CLN6 fusion protein Ag14159 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ceroid-lipofuscinosis, neuronal 6, late infantile, variant
别名 CLN6, HsT18960, nclf, Protein CLN6
计算分子量 311 aa, 36 kDa
观测分子量 30 kDa
GenBank蛋白编号BC010849
基因名称 CLN6
Gene ID (NCBI) 54982
RRIDAB_2878671
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLN6 antibody 20315-1-APDownload protocol
IHC protocol for CLN6 antibody 20315-1-APDownload protocol
IF protocol for CLN6 antibody 20315-1-APDownload protocol
IP protocol for CLN6 antibody 20315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Genet Metab

Novel mutations in CLN6 cause late-infantile neuronal ceroid lipofuscinosis without visual impairment in two unrelated patients.

Authors - Joseph J Chin
View Article