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CLN6 Polyclonal antibody

CLN6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20315-1-AP

Print datasheet

Synonyms

CLN6, HsT18960, nclf, Protein CLN6

验证数据展示

  • WB analysis of SH-SY5Y using 20315-1-AP

    WB analysis of SH-SY5Y using 20315-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20315-1-AP (CLN6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20315-1-AP

    IP experiment of HEK-293 using 20315-1-AP

    IP result of anti-CLN6 (IP:20315-1-AP, 4ug; Detection:20315-1-AP 1:300) with HEK-293 cells lysate 2560ug.

  • IHC staining of human colon using 20315-1-AP

    IHC staining of human colon using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 20315-1-AP

    IHC staining of human colon using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 20315-1-AP

    IF Staining of HEK-293 using 20315-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20315-1-AP (CLN6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20315-1-AP is CLN6 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CLN6 fusion protein Ag14159 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ceroid-lipofuscinosis, neuronal 6, late infantile, variant
Synonyms CLN6, HsT18960, nclf, Protein CLN6
Calculated Molecular Weight 311 aa, 36 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC010849
Gene Symbol CLN6
Gene ID (NCBI) 54982
RRIDAB_2878671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLN6 antibody 20315-1-APDownload protocol
IHC protocol for CLN6 antibody 20315-1-APDownload protocol
IF protocol for CLN6 antibody 20315-1-APDownload protocol
IP protocol for CLN6 antibody 20315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Genet Metab

Novel mutations in CLN6 cause late-infantile neuronal ceroid lipofuscinosis without visual impairment in two unrelated patients.

Authors - Joseph J Chin
View Article