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CLP1 Polyclonal antibody

CLP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14746-1-AP

Print datasheet

Synonyms

CLP1, hClp1, HEAB, Polynucleotide kinase Clp1

验证数据展示

  • WB analysis of mouse testis using 14746-1-AP

    WB analysis of mouse testis using 14746-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14746-1-AP targets CLP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CLP1 fusion protein Ag6479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)
Synonyms CLP1, hClp1, HEAB, Polynucleotide kinase Clp1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 41 kDa, 48 kDa
GenBank Accession NumberBC000446
Gene Symbol CLP1
Gene ID (NCBI) 10978
RRIDAB_2878076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92989
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLP1, also named as HEAB, is a 425 amino acid protein, which belongs to the Clp1 family. CLP1 as a polynucleotide kinase, that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. Human CLP1 is a component of the mRNA 3'end cleavage and polyadenylation machinery4,5 and studies in yeast have postulated a function for CLP1 in coupling mRNA 3' end processing with RNA Pol II transcriptional termination. CLP1 exists 2 isoforms with MV 41 kDa and 48 kDa.

实验方案

Product Specific Protocols
WB protocol for CLP1 antibody 14746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nucleic Acids Res

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Authors - Xiaodong Lu
View Article