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CLPS Recombinant antibody

CLPS Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241565H3

Cat no : 84224-2-RR

Print datasheet

Synonyms

Colipase, 241565H3

验证数据展示

  • WB analysis of rat pancreas using 84224-2-RR

    WB analysis of rat pancreas using 84224-2-RR

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 84224-2-RR (CLPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 84224-2-RR

    IHC staining of mouse pancreas using 84224-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 84224-2-RR

    IHC staining of mouse pancreas using 84224-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84224-2-RR (CLPS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84224-2-RR

    Affinity and Kinetic Characterization of 84224-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84224-2-RR against Human CLPS were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84224-2-RR targets CLPS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CLPS fusion protein Ag2321 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colipase, pancreatic
Synonyms Colipase, 241565H3
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC017897
Gene Symbol CLPS
Gene ID (NCBI) 1208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

实验方案

Product Specific Protocols
WB protocol for CLPS antibody 84224-2-RRDownload protocol
IHC protocol for CLPS antibody 84224-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols