验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | A549 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15337-1-AP targets CLPTM1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | CLPTM1 fusion protein Ag7538 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cleft lip and palate associated transmembrane protein 1 |
别名 | Putative lipid scramblase CLPTM1 |
计算分子量 | 62 kDa |
观测分子量 | 65-75 kDa |
GenBank蛋白编号 | BC004865 |
基因名称 | CLPTM1 |
Gene ID (NCBI) | 1209 |
RRID | AB_3669204 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O96005 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cleft lip and palate transmembrane protein 1 (CLPTM1), a multipass transmembrane protein, was identified due to the association of a chromosomal translocation with a cleft lip and palate (PMID: 9828125). CLPTM1 regulates epileptic seizures by modulating GABAAR-mediated inhibitory synaptic transmission (PMID: 36519412). Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma (PMID: 34586883).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CLPTM1 antibody 15337-1-AP | Download protocol |
IHC protocol for CLPTM1 antibody 15337-1-AP | Download protocol |
IP protocol for CLPTM1 antibody 15337-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |