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验证数据展示

  • WB analysis of A549 using 31050-1-AP

    WB analysis of A549 using 31050-1-AP

    WB result of CLPX antibody (31050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells.

  • WB analysis using 31050-1-AP

    WB analysis using 31050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31050-1-AP (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 31050-1-AP

    IHC staining of mouse stomach using 31050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31050-1-AP (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CLPX Polyclonal antibody

CLPX Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EPP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31050-1-AP targets CLPX in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CLPX fusion protein Ag34213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ClpX caseinolytic peptidase X homolog (E. coli)
别名 EPP2
计算分子量69 kDa
观测分子量63 kDa
GenBank蛋白编号NM_006660
基因名称 CLPX
Gene ID (NCBI) 10845
RRIDAB_3669833
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76031
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLPX antibody 31050-1-APDownload protocol
IHC protocol for CLPX antibody 31050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols