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验证数据展示

  • WB analysis of A549 using 31050-1-AP

    WB analysis of A549 using 31050-1-AP

    WB result of CLPX antibody (31050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLPX transfected A549 cells.

  • WB analysis using 31050-1-AP

    WB analysis using 31050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31050-1-AP (CLPX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 31050-1-AP

    IHC staining of mouse stomach using 31050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31050-1-AP (CLPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CLPX Polyclonal antibody

CLPX Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31050-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EPP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31050-1-AP targets CLPX in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CLPX fusion protein Ag34213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ClpX caseinolytic peptidase X homolog (E. coli)
Synonyms EPP2
Calculated Molecular Weight69 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberNM_006660
Gene Symbol CLPX
Gene ID (NCBI) 10845
RRIDAB_3669833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLPX antibody 31050-1-APDownload protocol
IHC protocol for CLPX antibody 31050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols