验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
23206-1-AP targets Claspin in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Claspin fusion protein Ag19637 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claspin homolog (Xenopus laevis) |
| 别名 | hClaspin, CLSPN |
| 计算分子量 | 1339 aa, 151 kDa |
| 观测分子量 | 240 kDa |
| GenBank蛋白编号 | BC137279 |
| 基因名称 | Claspin |
| Gene ID (NCBI) | 63967 |
| RRID | AB_2879232 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAW4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLSPN, also named as Claspin, is a 1339 amino acid protein, which belongs to the claspin family. CLSPN localizes in the nucleus and is highly expressed at S/G2 phases of the cell cycle. CLSPN is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. CLSPN plays a potentially critical role in replication checkpoint control in cells and mediating phosphorylation and activation of checkpoint protein kinases. The calculated molecular weight of CLSPN is 151 kDa, but the modified protein is about 220-240 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claspin antibody 23206-1-AP | Download protocol |
| IHC protocol for Claspin antibody 23206-1-AP | Download protocol |
| IF protocol for Claspin antibody 23206-1-AP | Download protocol |
| IP protocol for Claspin antibody 23206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells. | ||
Int J Oncol Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells. | ||
Cell Insight ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance |
