验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12788-1-AP targets Calsyntenin-1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Calsyntenin-1 fusion protein Ag3504 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calsyntenin 1 |
| 别名 | ALC ALPHA, Alcadein alpha, alcalpha1, alcalpha2, C terminal fragment 1 alpha, calsyntenin 1, Calsyntenin-1, CLSTN1, CS1, CSTN1, KIAA0911, PIK3CD, SAlc alpha CTF1 alpha, XB31alpha |
| 计算分子量 | 109 kDa |
| 观测分子量 | 109-130 kDa |
| GenBank蛋白编号 | BC033902 |
| 基因名称 | Calsyntenin-1 |
| Gene ID (NCBI) | 22883 |
| RRID | AB_2229723 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94985 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calsyntenin-1 antibody 12788-1-AP | Download protocol |
| IHC protocol for Calsyntenin-1 antibody 12788-1-AP | Download protocol |
| IP protocol for Calsyntenin-1 antibody 12788-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurosci The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites. | ||
Front Oncol Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer. | ||
Cardiovasc Drugs Ther Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats | ||
Cell Death Discov ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer | ||
J Cancer Res Clin Oncol Explore the alterations of downstream molecular pathways caused by ARID1A mutation/knockout in human endometrial cancer cells |