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CLSTN3 Recombinant antibody

CLSTN3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240865C10

Cat no : 83931-1-RR

Print datasheet

Synonyms

Alc-beta, alcbeta, Alcadein-beta, Alcadein beta, Alc beta

验证数据展示

  • WB analysis using 83931-1-RR

    WB analysis using 83931-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83931-1-RR (CLSTN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83931-1-RR

    Affinity and Kinetic Characterization of 83931-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83931-1-RR against Human CLSTN3 were performed. The affinity constant is 1.51 nM.

经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83931-1-RR is CLSTN3 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen CLSTN3 fusion protein Ag14897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calsyntenin 3
Synonyms Alc-beta, alcbeta, Alcadein-beta, Alcadein beta, Alc beta
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight130 kDa, 140 kDa
GenBank Accession NumberBC039075
Gene Symbol CLSTN3
Gene ID (NCBI) 9746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BQT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calsyntenins, also called alcadeins, are cadherin superfamily proteins first identified as synaptic proteins (PMID: 11161476; 12498782). Calsyntenins are type I transmembrane proteins with extracellular domains containing two cadherin repeats and an LNS (laminin, neurexin, sex hormone-binding globulin) domain (PMID: 24613359). Calsyntenin-3 (CLSTN3, also known as alcadein-beta) is a synapse-organizing protein that promotes the development of synapses. It is a postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (PMID: 25352602; 24613359).

实验方案

Product Specific Protocols
WB protocol for CLSTN3 antibody 83931-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols