验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CLTCL1 Ready-To-Use IHC Kit. CLTCL1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22283-1-AP targets CLTCL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | clathrin, heavy chain-like 1 |
| 别名 | CLTD, CLTCL, CLH-22, CLH22, Clathrin heavy chain on chromosome 22 |
| 计算分子量 | 187 kDa |
| 观测分子量 | 180-210 kDa |
| GenBank蛋白编号 | NM_007098 |
| 基因名称 | CLTCL1 |
| Gene ID (NCBI) | 8218 |
| RRID | AB_11183764 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P53675 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLTCL1, also called CLH22, CLTCL, and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross-reaction to CLTC.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CLTCL1 antibody 22283-1-AP | Download protocol |
| IF protocol for CLTCL1 antibody 22283-1-AP | Download protocol |
| IP protocol for CLTCL1 antibody 22283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J CHC22 clathrin recruitment to the early secretory pathway requires two-site interaction with SNX5 and p115 | ||
J Cell Biol CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.
| ||
Proteome Sci LC-MS-based quantitation of proteomic changes induced by Norcantharidin in MTB-Treated macrophages |
